In a standard Thin Layer Chromatography (TLC) experiment, the mobile phase solvent front advances ex — Qualitative and Quantitative Analysis Chemistry Question
Question
In a standard Thin Layer Chromatography (TLC) experiment, the mobile phase solvent front advances exactly $10.0 \text{ cm}$ from the baseline. If a target organic substance advances exactly $6.5 \text{ cm}$ from the baseline, its Retardation factor ($R_f$) is:
Answer: A
💡 Solution & Explanation
The Retardation factor ($R_f$) is defined strictly as the ratio of the distance moved by the substance to the distance moved by the solvent front. Therefore, $R_f = 6.5 \text{ cm} / 10.0 \text{ cm} = 0.65$.
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