In a Thin Layer Chromatography (TLC) experiment, the mobile phase (solvent front) advances exactly f — Qualitative and Quantitative Analysis Chemistry Question
Question
In a Thin Layer Chromatography (TLC) experiment, the mobile phase (solvent front) advances exactly $10.0 \text{ cm}$ from the base line. If the Retardation factor ($R_f$) of a target organic substance is determined to be $0.65$, calculate the total distance moved by the substance from the base line, expressed in millimeters (mm).
Answer: 65
💡 Solution & Explanation
The Retardation factor ($R_f$) is defined as the distance moved by the substance divided by the distance moved by the solvent front. Given $R_f = 0.65$ and solvent distance = $10.0 \text{ cm}$. Distance of substance = $0.65 \times 10.0 \text{ cm} = 6.5 \text{ cm}$. Converting to millimeters: $6.5 \text{ cm} \times 10 \text{ mm/cm} = 65 \text{ mm}$.
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