Two organic compounds, I and II, are being eluted by column chromatography. It is observed that the — Qualitative and Quantitative Analysis Chemistry Question
Question
Two organic compounds, I and II, are being eluted by column chromatography. It is observed that the adsorption of compound I is greater than the adsorption of compound II on the stationary phase. Which of the following is a mathematically and physically correct statement regarding their elution?
💡 Solution & Explanation
Since the adsorption of I is stronger than II, compound I is more firmly attached to the stationary phase and will move slowly. Compound II is less strongly attached, hence it moves faster with the mobile phase and travels a larger distance. Consequently, compound II will have a higher $R_f$ (Retardation factor) value than I.